The Importance of Calibration
The fundamental basis of immunochemical methods is that the analyte being measured in the sample should have the same characteristics as the analyte in the assay calibrator. The level of analyte in the sample is then determined by comparing its immunoreactivity with the specific antibodies to that of the calibrator with accuracy-based assigned target values.
With Lipoprotein(a) testing, due to the range of isoforms that are developed as a result of the variability of repeats from kringle 4 type 2, each laboratory must be able to calibrate to cover the complete isoform range. Calibrators used must be a true reflection of the isoforms present in the population. However, there are calibrators used in Lipoprotein(a) testing that do not cover the variations in Isotopes- this will lead to bias in results and, more importantly, inaccurate patient results.
If a sample has a higher level of kringle 4 type 2 repeats than in the calibrator, values will be overestimated. Similarly, if a sample has a lower level of kringle 4 type 2 repeats than in the calibrator, values will be underestimated. Very simply, by not providing a calibration with a range of Lipoprotein(a) levels from low to high, results will be inaccurate. The Randox Lipoprotein(a) calibrator solves these problems and offers a superior calibration product – click here to find out how.